Journal: bioRxiv
Article Title: Intensive Chemotherapy Induces Cardiotoxicity via Reverse Electron Transport
doi: 10.1101/2024.12.20.629711
Figure Lengend Snippet: ( A ) Basal oxygen consumption rate measured by Seahorse MitoStress Test in untreated H9C2 cells or those treated with iCT, malonate, or their combination (n=4) Brown-Forsythe and Welch ANOVA test: ***p<0,001. ( B ) Maximum oxygen consumption rate measured by Seahorse MitoStress Test in H9C2 either untreated or treated with iCT, malonate, or the combination of iCT and malonate (Combo) for 24 hours (n=4) Brown-Forsythe and Welch ANOVA test. ( C ) ATP production measured by Seahorse MitoStress Test in H9C2 cells either untreated or treated with iCT, malonate, or the combination of iCT and malonate (Combo) for 24 hours (n=6). Friedman Test, *p<0,05; **p<0,01. ( D ) Histogram of NAD + concentration in H9C2 cells, either untreated or treated with iCT, malonate, or their combination (n=6). One-way ANOVA. ( E ) Histogram of NADH concentration in H9C2 cells, either untreated or treated with iCT, malonate, or their combination (n=6). One-way ANOVA: **p<0,01. ( F ) Histogram of succinate concentration in H9C2 cells treated with iCT, Malonate, or the combination of Malonate and iCT, normalized to control cells (n=4). One sample t-test, *p<0,05; **p<0.01. ( G ) Kinetics and ( H ) Histogram quantification of Mitosox Green fluorescence representing mitochondrial ROS before and after pre-treating H9C2 cells with malonate, followed by iCT treatment, or after iCT treatment alone (n=6). One-way ANOVA: *p<0.05; **p<0.01 ( I ) Histogram of viable H9C2 cell counts, comparing untreated cells to those treated with iCT, S3QEL2, or a combination of iCT and S3QEL2, measured with a Malassez counting chamber (n=6). Brown-Forsythe and Welch ANOVA test: **p<0,01. ( J ) Kinetics of Mitosox Green fluorescence in H9C2 treated or not with iCT, S3QEL2, or a combination of S3QEL2 and iCT. One-way ANOVA: ns; not significant. ( K ) Histogram of viable H9C2 cell counts, comparing untreated cells to those treated with iCT, S1QEL1, or a combination of iCT and S1QEL1, measured with a Malassez counting chamber (n=6). Brown-Forsythe and Welch ANOVA test: *p<0,05; ***p<0,001. ( L ) Kinetics of Mitosox Green fluorescence before and after pre-treating H9C2 cells with S1QEL1 followed by iCT treatment, or after iCT treatment alone (n=5) One-way ANOVA: *p<0.05.
Article Snippet: To measure mitochondrial ROS production, fluorescence intensity was monitored using the Incucyte SX G/O/NIR Optical Module (Sartorius) with a 20X objective.
Techniques: Concentration Assay, Control, Fluorescence
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